Figure 5.

TIBA binds to VLN4. A, Diagram of VLN4 truncated fragments. B, Thermophoresis binding curves of fragmented VLN4 proteins titrated with TIBA from experiments of three biological replicates. C, The drug affinity responsive target stability (DARTS) assay shows that TIBA protects VLN4, but not actin, from degradation. Top, Western blot of DARTS samples treated with different concentrations of pronase using anti-HA (Human influenza hemagglutinin) and antiactin antibodies. Bottom, Quantification of the protein band intensity between TIBA and dimethyl sulfoxide (DMSO) reveals that the presence of TIBA stabilizes VLN4 in cells. Values given are means ± se (n = 3 independent experiments; *P < 0.05; **P < 0.01 between TIBA and DMSO treatment; t test).