Table 1. Single filament dynamics in wild-type treated with mock or 10 µm TIBA.
Stochastic Dynamics Parameters | Mock | TIBA |
---|---|---|
Elongation rate (µm/s) | 1.5 ± 0.1 | 1.8 ± 0.1** |
Max. filament length (µm) | 7.1 ± 0.3 | 9.6 ± 0.5*** |
Max. filament lifetime (s) | 14.7 ± 1.3 | 23.1 ± 1.7*** |
Severing frequency (breaks/µm/s; 10−2) | 3.1 ± 0.2 | 1.8 ± 0.2*** |
Regrowth frequency (%) | 2.4 ± 0.1 | 2.2 ± 1.1 (nd) |
Annealing frequency (%) | 11.7 ± 3.9 | 16.4 ± 3.6 (nd) |
Bundling frequency (events/µm2/filament/s; 10−6) | 6.1 ± 1.1 | 17.4 ± 2.3*** |
Debundling frequency (events/µm2/filament/s; 10−6) | 1.7 ± 0.6 | 2.3 ± 0.9 (nd) |