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. 2019 Jul 9;181(1):262–275. doi: 10.1104/pp.19.00610

Figure 6.

Figure 6.

ROS generation in mid-aged leaves under −Mg stress. A, Phenotypic profile of leaf blades (left) and leaf sheaths (right) of mid-aged leaves. Both wild-type (WT) and mutant rice seedlings were grown in nutrient solution containing 0- or 250-μm Mg for 8 d. Scale bars = 1 cm. B and C, Comparison of Fv/Fm values in different Mg deficient times (B) and different leaves (C). Rice seedlings were dark-adapted overnight. The maximum efficiency of PSII photochemistry Fv/Fm = (Fm − F0)/Fm, was determined by a portable photosynthesis system (model no. LI-6800; Li-Cor). Data represent means ± sd (n = 3). D, CM-H2DCFDA and DHE staining of ROS intermediates in leaf blades. Mid-aged leaves were incubated in 10-μm CM-H2DCFDA or 40-μm DHE for 30 min and imaged by confocal microscopy. Scale bars = 100 μm. E and F, Relative intensity of CM-H2DCFDA (E) and DHE (F) staining. Data represent means ± sd (n = 6). The asterisk in (B) and (C) shows a significant difference compared to wild type (P < 0.05 by Tukey’s test). Means with different letters in (E) and (F) are significantly different (P < 0.05 by Tukey’s test).