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. 2019 Aug 23;13:56. doi: 10.3389/fncir.2019.00056

FIGURE 1.

FIGURE 1

Somatostatin-expressing hilar interneurons make axonal projections to the contralateral hippocampus. (A) Schematic of the experimental approach. (B) Localized expression of AAV1-FLEX-CAG-GFP in the injected brain hemisphere after stereotaxic injection into the dentate gyrus hilus region of a GAD2-cre mouse. Coronal section. Single confocal plane. Scale bar = 100 μm. (C) Contralateral hippocampus of the same section shown in (B). Note the lack of somatic labeling, but labeling of axons, particularly in the dentate molecular layers. Single confocal plane. Scale bar = 100 μm. (D) Localized expression of AAV1-FLEX-CAG-GFP in the injected brain hemisphere after stereotaxic injection into the dentate gyrus hilus region of a SOM-cre mouse. Coronal section. Single confocal plane. Scale bar = 200 μm. (E–G) GFP was expressed in somata of the ipsilateral hilus (G), but not in CA3 (E) or CA1 (F). Single confocal sections. Scale bars = 10, 50, and 50 μm. (H) Low magnification image of the contralateral hemisphere of the same section shown in (D), indicating a lack of somatic GFP. Single confocal section. Scale bar = 200 μm. (I) High magnification image of a GFP-labeled axon in the contralateral hilar region. Maximum intensity projection of 28 planes (1 μm spacing). Scale bar = 20 μm. (J,K) Viral GFP-expressing axons in the ipsilateral Infragranular molecular layer, either as a single confocal section (J) or the same field of view as a maximum intensity projection of 25 planes (K, 1 μm spacing). Scale bars = 10 μm. (L,M) Viral GFP-expressing axons in the contralateral Infragranular molecular layer, either as a single confocal section (L) or the same field of view as a maximum intensity projection of 25 planes (M, 1 μm spacing). Scale bars = 10 μm.