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. Author manuscript; available in PMC: 2019 Aug 30.
Published in final edited form as: Nat Protoc. 2019 May 17;14(6):1772–1802. doi: 10.1038/s41596-019-0161-7

Fig. 9 |. Schematics of FIB-SEM workflow.

Fig. 9 |

a, Cells cultured on a nanochip, b, Cells fixed in glutaraldehyde after culture, processed with the RO-T-O procedure and uranyl acetate to increase their contrast, c, Cells on a nanochip embedded in resin, d, resin excess removal and subsequent polymerization. To remove the resin excess, the sample is placed in a vertical position and gently wash them with ethanol, e, Cells on nanochip coated with Au. f, Pt deposition assisted by both E-beam and I-beam, g, I-beam induced cross sectioning, h, Imaging the cell-nanostructure interface after cross-sectioning.