KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-HA-Peroxidase	Roche	Cat # 12013819001; RRID:AB_439705
Anti-FLAG-Peroxidase	Sigma-Aldrich	Cat # A8592; RRID:AB_259529
Anti-GFP	Roche	Cat # 11814460001; RRID:AB_390913
Anti-Mouse IgG HRP-linked antibody	Cell Signaling	Cat # 7076; RRID:AB_330924
Anti-FLAG M2 Affinity gel	Sigma-Aldrich	Cat # 2220; RRID:AB_10063035
Protein G Agarose	Roche	Cat #5015952001
Bacterial and Virus Strains
E.coli MC1061	Li et al., 2015	N/A
Agrobacterium tumefaciens GV3101	Li et al., 2015	N/A
E. coli BL21	Li et al., 2015	N/A
Pseudomonas syringae pv. tomato DC3000 (Pst)	He et al., 2006	N/A
P. syringae pv. maculicola ES4326 (Psm)	Li et al., 2014a	N/A
Pseudomonas syringae pv. tomato DC3000 (Pst) type III secretion mutant hrcC	He et al., 2006	N/A
Chemicals, Peptides, and Recombinant Proteins
PD184161	A.G. Scientific	Cat # P-1713
Calf intestinal alkaline phosphatase (CIP)	New England BioLabs	Cat # M0290S
Lambda protein phosphatase (λPP)	New England BioLabs	Cat # P0753S
Cordycepin	Sigma-Aldrich	Cat # C9137
RiboZol RNA Extraction Reagent	AMRESCO	Cat # N580
RNase-free DNase I	New England BioLabs	Cat # M0303L
flg22	Genscript	N/A
elf18	Genscript	N/A
chitin	Sigma-Aldrich	Cat # C9752
peptidoglycan	Sigma-Aldrich	Cat #77140
lipopolysaccharide	Sigma-Aldrich	Cat # L2012
IPTG	Sigma-Aldrich	Cat# I6758
Pierce glutathione agarose	Thermo Scientific	Cat# 16101
amylose resin	New England BioLabs	Cat# E8021L
Critical Commercial Assays
M-MuLV Reverse Transcriptase	New England BioLabs	Cat # M0253L
iTaq SYBR green Supermix	Bio-Rad	Cat # 1725124
Experimental Models: Organisms/Strains
Arabidopsis thaliana Col-0 wild-type	Li et al., 2015	N/A
Arabidopsis thaliana Ler ecotype	Li et al., 2015	N/A
mpk3	ABRC	SALK_151594
mpk6	ABRC	Salk_073907
mpk4	ABRC	CS5205, Ler background
fls2	ABRC	Salk_141277
dcp1–1	ABRC	GABI-844B03
p35S::DCP1-GFP/Col-0	This paper	N/A
p35S::DCP1S237A-GFP/Col-0	This paper	N/A
pDCP1::DCP1-GFP/dcp1–1	This paper	N/A
pDCP1::DCP1S237A-GFP/dcp1–1	This paper	N/A
pDCP1::DCP1S237D-GFP/dcp1–1	This paper	N/A
mpk6/amiR-MPK3	This paper	N/A
Oligonucleotides
Primers for cloning, point mutation and VIGS, see Table S1	This paper	N/A
Primers for genotyping and RT-PCR, see Table S1	This paper	N/A
Primers for qRT-PCR, see Table S1	This paper	N/A
Recombinant DNA
pYL156 (pTRV-RNA2)	Li et al., 2014a	N/A
pTRV-RNA1	Li et al., 2014a	N/A
pYL156-GFP	Li et al., 2014a	N/A
pHBT	He et al., 2006	N/A
pGST	Lu et al., 2010	N/A
pMAL-c2	Lu et al., 2010	N/A
pCB302	Li et al., 2014a	N/A
pHBT-amiRNA-ICE1	Li et al., 2014b	N/A
pTA7002-AvrPto	He et al., 2006	N/A
pYL156-DCP1	This paper	N/A
pYL156-DCP2	This paper	N/A
pHBT-DCP1-GFP	This paper	N/A
pHBT-DCP1-HA	This paper	N/A
pHBT-DCP1-FLAG	This paper	N/A
pHBT-DCP2-GFP	This paper	N/A
pHBT-DCP2-FLAG	This paper	N/A
pHBT-XRN4-GFP	This paper	N/A
pHBT-XRN4-HA	This paper	N/A
pHBT-XRN4-FLAG	This paper	N/A
pHBT-DCP5-GFP	This paper	N/A
pHBT-DCP5-HA	This paper	N/A
pHBT-PAT1-GFP	This paper	N/A
pHBT-DCP1S237A-HA	This paper	N/A
pHBT-DCP1S237D-HA	This paper	N/A
pHBT-MEKK1-FLAG	Li et al., 2015	N/A
pHBT-MPK3-FLAG	Li et al., 2014a	N/A
pHBT-MPK6-FLAG	Li et al., 2014a	N/A
pCB302–35S::DCP1-GFP	This paper	N/A
pCB302–35S::DCP1 S237A-GFP	This paper	N/A
pCB302–35S::DCP1 S237D-GFP	This paper	N/A
pCB302–35S::DCP2-FLAG	This paper	N/A
pCB302–35S::XRN4-HA	This paper	N/A
pCB302–35S::MPK3-FLAG	This paper	N/A
pCB302–35S::MPK6-FLAG	This paper	N/A
pCB302-pDCP1::DCP1-GFP	This paper	N/A
pCB302-pDCP1::DCP1 S237A-GFP	This paper	N/A
pCB302-pDCP1::DCP1 S237D-GFP	This paper	N/A
pMAL-DCP1	This paper	N/A
pMAL-DCP1 S237A	This paper	N/A
pMAL-DCP1 S237D	This paper	N/A
pGST-DCP2	This paper	N/A
pGST-XRN4	This paper	N/A
pGST-MPK3	This paper	N/A
pGST-MPK6	This paper	N/A
pTA7002-amiR-MPK3	This paper	N/A
Software and Algorithms
Olympus Fluoview Viewer	Olympus	Version 3.0
ImageJ	NIH	https://imagej.nih.gov/ij/