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. 2019 Aug 23;7:161. doi: 10.3389/fcell.2019.00161

FIGURE 2.

FIGURE 2

miR-455-3p and HOTTIP regulate the expression of CCL3 and cartilage-specific gene during hMSCs chondrogenesis. hMSCs were transfected with miR-control, miR-455-3p, pcDNA3.1-HOTTIP, pcDNA3.1-HOTTIP + miR-455-3p, anti-miR-control, anti-miR-455-3p, siHOTTIP, siHOTTIP + anti-miR-455-3p, respectively, and then induced to differentiate into chondrocytes for 14 days. The expression level of miR-455-3p (A,L) and HOTTIP (B,M) were estimated by RT-qPCR, while the expression levels of CCL3 (C,K,N,V) and Col2a1 (D,K,O,V), Aggrecan (E,K,P,V), SOX9 (F,K,Q,V), MMP3 (G,K,R,V), MMP13 (H,K,S,V), RUNX2 (I,K,T,V), and COL10A1 (J,K,U,V) were estimated by RT-qPCR and western blotting. GAPDH and β-actin were used as endogenous controls. The data shown represent the mean ± SD of three independent experiments performed with samples from three different donors. *p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.