Fig 6. Protective effects of the flavodoxins on macromolecules damage of P. xenovorans after exposure to oxidizing agents paraquat and H₂O₂.

(A) For protein carbonylation, nucleic acid-free protein extracts were derivatized with DNPH. After addition of 6 M NaOH, the product was measured spectrophotometrically. (B) Lipid peroxidation was measured by the thiobarbituric acid reactive substances (TBARS) assay. Samples were incubated with TBA for 1 h at 100°C and measured spectrophotometrically. Significant differences were determined with a Tukey Test (p<0.05) as indicated. Lower case letter under the error bars indicate significant differences between strains in each treatment. Capital letter under the error bars indicate significant differences between treatments for each strain. Each value is an average ± SD of at least three independent experiments.