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. 2019 Aug 20;15(8):e1008325. doi: 10.1371/journal.pgen.1008325

Fig 4. NKILA represses the tumorigenesis of NPC.

Fig 4

(A) Apoptosis of control vector or NKILA-overexpressing S18 cells and shGFP or shNKILA S26 cells as assayed by ANNEXIN-V/PI staining(mean±SD, n = 3,***<0.001 versus S26 shGFP,**<0.01versus S18 vec). (B) Tunnel assay revealing the correlation between NKILA and apoptosis in 65 paraffin-embedded NPC specimens. (C) Anchorage-independent growth of control vector or NKILA-overexpressing S18 cells and shGFP or shNKILA S26 cells in soft agar. Over 14 days of culture, number of colonies was calculated at 10 randomly ten fields of view. Original magnification, ×100. (Error bar represent SD. Mean ± SD, n = 3, ***, P < 0.001 versus S18 vec; ###, P < 0.001 versus S26 shGFP). (D) Foci formation by control vector or NKILA-overexpressing S18 cells and shGFP or shNKILA S26 cells were performed as described in the Methods. (E) Tumor formation in nude mice: S18 (vec, NKILA) cells were injected and tumor volumes were calculated and plotted as described in the Methods. The upper panel shows the tumor growth and tumor volume in mice (S18 vector control, S18 NKILA overexpressing cells). The tumor weight is also shown in the lower panel, and tumors treated with vec are much heavier, indicating that NKILA can inhibit tumor growth.