Figure 4:

2D ¹H,¹⁵N-HSQC NMR titrations of PAR1G_ED (49–62) in the presence of PPACK-IIa and GpIbα3P – PPACK-IIa. For these 2D NMR studies, the initial (PPACK-IIa or GpIbα3P – PPACK-IIa) to PAR1G_ED (49–62) ratio was 4:1 or 3:1 (blue) and then gradually diluted down to 0.1:1 (red). A) 2D ¹H,¹⁵N-HSQC NMR titrations for 50 μM PAR1G_ED (49–62, [¹⁵N]-E53, [¹⁵N]-D58) in 200μM PPACK-IIa. B) ¹H,¹⁵N-2D HSQC of 50 μM PAR1G_ED (49–62, [¹⁵N]-E53, [¹⁵N]-D58) in 150 μM GpIbα3P – PPACK-IIa.