Figure 6:

1D, and 2D ¹H,¹⁵N-HSQC NMR titrations of PAR3G_EL (44–56) with GpIbα3P – PPACK-IIa. For these NMR studies, the initial GpIbα3P – PPACK-IIa to PAR3G_EL (44–56) ratio was 3:1 and then gradually diluted down to 0.1:1. A) 1D 1H,15N-HSQC NMR titrations for starting concentrations of 50 μM PAR3G_EL (44–56, [¹⁵N]-E48, [¹⁵N]-L52) in at least 150 μM GpIbα3P – PPACK-IIa. B) 2D ¹H,¹⁵N-HSQC NMR titrations of 50 μM PAR3GEL (44–56, [¹⁵N]-E48, [¹⁵N]-L52) in at least 150 μM GpIbα3P – PPACK-IIa.