Fig. 2.

Higher STIM2 protein expression levels are associated with human breast cancer metastasis. a Representative images of immunohistochemical (IHC) staining (× 200) of STIM1 and STIM2 in mammary gland, carcinoma in situ, invasive carcinoma, and lymph node metastasis tissue microarrays. b, c Quantification of IHC staining intensity of STIM2 (b) and STIM1 (c) in breast cancer and normal mammary tissues. The staining intensity was scored with grades 0–3, and the data were analyzed with GraphPad Prism software version 6. Each symbol represents an individual sample. Statistical comparisons of staining intensity were performed using Kruskal-Wallis tests. d, e, f Representative Western blots (d) and quantification (e, f) of the protein expression levels of STIM1 and STIM2 in 5 breast cancer cell lines. g, h Images (× 20) (g) and quantification (h) of Transwell assays showing migration of 5 breast cancer cell lines. T tests or one-way ANOVA were used to compare independent groups. Data shown are means ± SD of at least 3 independent experiments. *P < 0.05