Fig. 4.

STIM2 regulates the expression and nuclear translocation of transcription factor NFAT1. a qRT-PCR analysis of the expression of transcription factors in MDA-MB-231 cells with STIM2 (top) or STIM1 (bottom) knockdown (SH) or overexpression (OE). b qRT-PCR analysis of the expression of NFAT1 in MCF7 cells with STIM2 knockdown or overexpression. c Western blots (left) and quantification (right) of NFAT1 protein levels in MDA-MB-231 cells with STIM2 or STIM1 knockdown or overexpression. d Western blots (left) and quantification (right) of NFAT1 protein levels in MCF7 cells with STIM2 knockdown or overexpression. e Western blots (left) and quantification (right) showing NFAT1 levels in the nuclear extracts of a panel of breast cancer cell lines. f Immunofluorescent staining (× 100) of NFAT1 in MDA-MB-231 cells with STIM2 knockdown or overexpression. One-way ANOVA was used to compare independent groups. Data shown are means ± SD of at least 3 independent experiments. *P < 0.05