Fig. 1.

Schematic diagram of genotypes of R. eutropha NSDG-GG-based recombinant strains. phaC_NSDG, a gene encoding N149S/D171G mutant of PHA synthase from A. caviae; phaA, β-ketothiolase gene; phaB1, a gene encoding major NADPH-acetoacetyl-CoA reductase; phaR_Re, PHA-binding transcriptional repressor gene; phaB1_NV and phaB1_NVYF, genes encoding N142V and N142V/Y185F mutants of PhaB1; phaB2, a gene encoding minor NADPH-acetoacetyl-CoA reductase; had, NAD-(S)-3HB-CoA dehydrogenase gene, crt2, crotonase gene. The commonly modified loci in these strains are shown in Additional file 1: Fig. S1