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. 2019 Aug 29;18:147. doi: 10.1186/s12934-019-1197-7

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Proposed pathway for P(3HB-co-3HHx) biosynthesis from glucose by R. eutropha NSDG-GG-HC/pBPP-ccr_MeJ_Ac-emd. PhaA and BktB, β-ketothiolases; PhaB1 and PhaB3, NADPH-acetoacetyl-CoA reductases; Had, NAD-(S)-3HB-CoA dehydrogenase; Crt2, crotonase; PhaC_NSDG, N149S/D171G mutant of PHA synthase from A. caviae; PhaJ_Ac, short-chain-length-(R)-enoyl-CoA hydratase from A. caviae; Ccr_Me, crotonyl-CoA carboxylase/reductase from Methylorubrum extorquens; Emd_Mm, ethylmalonyl-CoA decarboxylase from Mus musculus