Fig. 3.

Representative photomicrographs of pre-OLs a–f by immunohistochemistry using PDGFα-R and NG2 and O4+ oligodendrocyte (g–i) and TUNEL staining cells j–l by immunofluorescence (IF) staining of the cingulum (Bregma − 1.0) on P4. Pre-OLs were attenuated in the L group and preserved in the LE group (p, q). Double-labelling showed that many TUNEL-positive cells were also positive for O4 (m–o). O4+ oligodendrocytes were decreased in the L group with increased TUNEL-positive cells in the IF staining, and O4+ oligodendrocytes were recovered in the LE group (r–t). ** and *represent significant differences between groups (P < 0.01 and P < 0.05, respectively). ANOVA with a Bonferroni test was conducted. There were six rats per group