Table 3.
Physical properties of identified β-defensin peptides
| Defensin domain | Length | Molecular weight | Cys Residue Spacing | Charge | Isoelectric point |
|---|---|---|---|---|---|
| VkBD1_VARKO | 41 | 4816.46 | 6–3–9-6 | 4.5 | 7.9826 |
| VkBD2_VARKO | 44 | 4770.49 | 6–4–9-6 | 4 | 8.5177 |
| VkBD3_VARKO | 68 | 7510.69 | 6–4–9-6 | -1 | 5.635 |
| VkBD4_VARKO | 48 | 5466.13 | 6–4–9-6 | −0.5 | 6.1876 |
| VkBD5_VARKO | 45 | 5112.8 | 6–4–9-6 | 4.5 | 8.5056 |
| VkBD6_VARKO | 70 | 7548.7 | 6–4–9-7-7 − 5 | 4.5 | 7.9825 |
| VkBD7_VARKO | 83 | 8922.43 | 6–6–8-5/6–6–7-5 | 6 | 8.2233 |
| VkBD8_VARKO | 39 | 3850.54 | 6–6–7-5 | 2.5 | 7.9391 |
| VkBD9_VARKO | 46 | 5087.15 | 6–6–9-5 | 7.5 | 9.7437 |
| VkBD10_VARKO | 49 | 5346.37 | 6–6–7-5 | 0.5 | 6.8341 |
| VkBD11_VARKO | 40 | 4130.68 | 6–7–9-5 | -5 | 3.8346 |
| VkBD12_VARKO | 61 | 7362.5 | 6–6–12-7 | 6 | 9.0239 |
| VkBD13_VARKO | 56 | 6373.34 | 6–6–12-7 | 2 | 7.9229 |
| VkBD14_VARKO | 53 | 6051.22 | 6–6–12-7 | 9.5 | 10.3828 |
| VkBD15_VARKO | 42 | 4598.66 | 6–6–9-6 | 9.5 | 10.246 |
| VkBD16_VARKO | 54 | 5598.25 | 6–2–3-10-3-3 | 2 | 7.796 |
| VkBD17_VARKO | 47 | 5538.28 | 6–2–3-9-3-3 | 6 | 8.4828 |
| VkBD18_VARKO | 50 | 5606.31 | 6–2–3-10-3-3 | 2.5 | 7.512 |
| VkBD19_VARKO | 52 | 5871.76 | 7–6–10-7 | −0.5 | 5.6992 |
| VkBD20_VARKO | 43 | 4861.77 | 1–3–9-7 | 2 | 7.9237 |
| VkBD21_VARKO | 38 | 4009.73 | 6–4–9-5 | 6.5 | 9.1233 |
| VkBD22_VARKO | 42 | 4645.55 | 6–4–9-5 | 4.5 | 8.5176 |
| VkBD23_VARKO | 104 | 11,026.09 | 6–4–9-6 | −3 | 4.6839 |
| VkBD24_VARKO | 48 | 5012.86 | 7–4–9-6 | 3.5 | 8.2461 |
| VkBD25_VARKO | 42 | 4575.41 | 6–4–9-5 | 3.5 | 8.2423 |
| VkBD26_VARKO | 44 | 4914.62 | 6–4–11-5 | −3 | 4.2446 |
| VkBD27_VARKO | 41 | 4535.29 | 6–4–10 − 5 | 7 | 9.6914 |
| VkBD28_VARKO | 45 | 4618.16 | 6–4–9-6 | 0 | 5.9979 |
| VkBD29_VARKO | 44 | 4958.8 | 6–7–9-5 | 4.5 | 8.5135 |
| VkBD30_VARKO | 44 | 4538.29 | 6–8–9-5 | 0 | 6.0306 |
| VkBD31_VARKO | 44 | 5009.92 | 6–7–10-5 | 3 | 8.1509 |
| VkBD32_VARKO | 41 | 4339.01 | 6–4–9-5 | 3.5 | 8.1572 |
| VkBD33_VARKO | 44 | 4782.74 | 6–4–9-5 | 8.5 | 10.5052 |
| VkBD34_VARKO | 84 | 9330.95 | 7–4–9-5/7–4–9-5 | 4.5 | 8.0587 |
| VkBD35_VARKO | 47 | 5058.7 | 6–5–9-5 | -5 | 3.9254 |
| VkBD36_VARKO | 48 | 5026.65 | 6–5–9-5 | −8 | 3.371 |
| VkBD37_VARKO | 43 | 4702.36 | 6–4–9-5 | −1 | 4.7382 |
| VkBD38_VARKO | 44 | 5172.09 | 6–4–10-6 | 2.5 | 7.9416 |
| VkBD39_VARKO | 111 | 11,483.56 | 6–4–9-5/6–4–9-5/6–4–9-5 | 5 | 7.86 |
| VkBD40_VARKO | 43 | 4914.68 | 6–4–9-5 | 2.5 | 7.9327 |
| VkBD41_VARKO | 41 | 4641.48 | 6–4–8-5 | 3.5 | 7.9625 |
| VkBD42_VARKO | 42 | 4717.47 | 6–4–8-5 | 5.5 | 8.8017 |
| VkBD43_VARKO | 74 | 7874.1 | 6–4–9-5/6–4–9-5 | 2.5 | 7.65 |
| VkBD44_VARKO | 45 | 5073.96 | 6–4–9-5 | 5 | 8.5147 |
| VkBD45_VARKO | 38 | 4229.98 | 6–3–9-5 | 8 | 9.7441 |
| VkBD46_VARKO | 40 | 4388.17 | 6–4–9-5 | 9.5 | 10.8323 |
| VkBD47_VARKO | 44 | 5072.96 | 6–4–10-5 | 6.5 | 9.154 |
| VkBD48_VARKO | 52 | 5934.01 | 6–4–9-5 | 13 | 11.9504 |
| VkBD49_VARKO | 40 | 4566.32 | 6–4–9-5 | 5.5 | 8.814 |
| VkBD50_VARKO | 42 | 4627.4 | 6–4–9-5 | 7 | 10.4698 |
| VkBD51_VARKO | 40 | 4467.32 | 6–4–9-5 | 9 | 11.3419 |
| VkBD52_VARKO | 40 | 4623.65 | 6–4–9-5 | 8 | 11.1693 |
| VkBD53_VARKO | 40 | 4537.41 | 6–4–9-5 | 10 | 11.9738 |
| VkBD54_VARKO | 42 | 4760.65 | 6–4–9-5 | 11 | 12.1412 |
| VkBD55_VARKO | 56 | 6092.22 | 6–2–9-6 | 6.5 | 8.7963 |
| VkBD56_VARKO | 44 | 5031.94 | 3–4–8-5 | 8.5 | 9.2085 |
| VkBD57_VARKO | 40 | 4852.66 | 6–4–9-5 | 8.5 | 9.9206 |
| VkBD58_VARKO | 44 | 5254.16 | 6–7–8-5 | 6.5 | 8.8379 |
| VkBD59_VARKO | 40 | 4369.02 | 6–4–9-5 | 5.5 | 8.6986 |
| VkBD60_VARKO | 44 | 5232 | 6–4–10-5 | 6.5 | 8.6105 |
| VkBD61_VARKO | 44 | 5146.91 | 6–4–9-6 | 7 | 9.5242 |
| VkBD62_VARKO | 43 | 4829.59 | 3–10–16-6 | 7.5 | 9.1865 |
| VkBD79_VARKO | 49 | 5260.29 | 6–4–9-6 | 4 | 8.2526 |
| VkBD80a_VARKO | 45 | 5112.96 | 6–4–9-6 | 5.5 | 8.5212 |
| VkBD80b_VARKO | 45 | 5166 | 6–4–9-6 | 6.5 | 9.0493 |
| VkBDic1_VARKO | 44 | 5069.05 | 6–4–9-5 | 10 | 10.3026 |
Molecular weights, charges and isoelectric points were calculated based on predicted peptide sequences using the EMBOSS tool pepstats [53]