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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Psychoneuroendocrinology. 2019 Apr 6;106:244–251. doi: 10.1016/j.psyneuen.2019.04.007

Figure 2.

Figure 2.

Expression of oxytocin, oxytocin receptor and interleukin-6 in SKOV-3, OVCAR-8 and HEYA8 human ovarian cancer cell lines. Panels A-C, mRNA expression of oxytocin, oxytocin receptor and interleukin −6 for each of the cell lines studied; mRNA levels are expressed as relative quantitation (RQ) and were arbitrarily normalized to levels of SKOV-3 cells. Panel D, oxytocin receptor protein expression in replicate samples of SKOV-3, OVCAR-8 and HEYA8 cells evaluated by Western blotting, demonstrating two immunoreactive bands representing a lower unglycoslyated 43 kDa OTR and a 57 kDa glycosylated receptor. Panel E, mean expression level (in arbitrary units) of each band normalized for actin. Panel F and G, supernatant interleukin-6 and oxytocin protein levels after a six-hour incubation (ng of I interleukin-6 per mg of cell protein or pg of oxytocin/mg of cell protein). * indicates p <0.001 comparing expression levels of HEYA8 or OVCAR-8 to SKOV-3 cells and † indicates p <0.02 comparing HEYA8 to OVCAR-8.