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. 2019 Aug;60(10):3659–3668. doi: 10.1167/iovs.18-25118

Figure 1.

Figure 1

Retinal neurites grow directionally toward the cathode. Retinal explants were grown overnight, then exposed to an EF for 4 hours. (A) Control culture. (B) Culture exposed to EF of 200 mV/mm (cathode on right; anode on left). Left: Composite image of culture. Right: Tracing of explant and all neurites projecting from explant edge. (C, D) Composite of traced neurites from (A, B). See Methods. (E) Schematic of neurite quantification algorithm: gray: anode facing, purple: cathode facing, blue: perpendicular to EF. (F) Retinal explants were exposed to varying strengths of EFs. Neurites projecting from explant edge were quantified as schematized in (E) and represented as percent of total neurites growing toward cathode or anode versus perpendicular to EF. Significantly more neurites demonstrated cathode-directed growth when exposed to an EF of 100 or 200 mV/mm than 0 or 50 mV/mm (*; **; ***; ****P < 0.001; 2-way ANOVA). Error bars represent standard deviation (SD). Scale bar: 100 μm.