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. 2019 Aug;60(10):3659–3668. doi: 10.1167/iovs.18-25118

Figure 6.

Figure 6

Toxin B partially neutralizes cathode-directed growth of RGC axons. Explants were grown for 12 hours in varying concentrations of toxin B, then exposed to an EF of 200 mV/mm. Toxin B was replenished 1 hour before initiating EF exposure. (A) Significantly fewer axons were observed to grow toward the cathode in explants exposed to 1 ng/mL (*P < 0.001; 2-way ANOVA with Tukey's multiple comparison test) and 10 ng/mL (**P < 0.05; 2-way ANOVA with Tukey's multiple comparison test). No effect was noted when 0.1 or 0.5 ng/mL toxin B was added to culture. Error bars represent SD. (B) The effect of 1 and 10 ng/mL toxin B on rate of axon growth, with and without an EF, was quantified. Toxin B had no significant effect on rate of axon growth. Error bars represent SD.