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. Author manuscript; available in PMC: 2019 Oct 24.
Published in final edited form as: Lab Invest. 2019 Apr 24;99(9):1376–1388. doi: 10.1038/s41374-019-0259-0

Figure 4. I/R mediated injury changes the pattern of VEGF-C and VEGF-D expression and induces expression of VEGF-R3.

Figure 4.

(A) Mice were sacrificed at indicated time points and kidney sections were prepared and immunofluorescence assays were performed using anti-VEGF-C and anti-VEGF-D antibodies to determine the pattern of expression of these proteins. DAPI was used to stain nuclei. Images are representative of five independent experiments. Scale bar= 50 μm. (B) Kidneys sections were stained using anti-VEGF-R3 antibody. For better demonstration only the indicated time-points are shown. Scale bar= 50 μm. (C) Quantification of VEGF-R3 positive vessels was performed as described in methods. *P < 0.05 vs. baseline.