Figure 8. DT induced proximal tubular injury affects expression of LA ligands and leads to induction of LA.

(A) DT was administered to transgenic mice with selective expression of DT-receptor in proximal tubules and kidneys were harvested. Kidney sections were prepared and immunofluorescence staining was performed using anti-VEGF-C and anti-VEGF-D antibodies. DAPI was used to stain the nuclei. Images are representative of at least five independent experiments. Scale bar= 50 μm. (B) Kidney sections were stained with anti-VEGF-R3 antibody and nuclei were stained with DAPI. (C) Each stained kidney section was carefully evaluated to quantify the number of lymphatic vessels staining positive for VEGF-R3 and data was expressed as VEGF-R3 vessels per section. Images are representative of at least five independent experiments. Scale bar= 50 μm. Results expressed as mean ± SEM. *P < 0.05