1.
Key Resources Table
| Reagent and Resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Mouse anti-tyrosine hydroxylase | Santa Cruz | sc-25269 |
| Rabbit anti-MTTP | Abcam | ab186446 |
| Mouse anti-CPT1a | Abcam | ab128568 |
| Rabbit anti-ACOX1 | Abcam | ab184032 |
| Rabbit anti-GAPDH | Abcam | ab181602 |
| Rabbit anti-CD36 | Novus Biologicals | NB400–144 |
| Mouse anti-citrate synthase | Novus Biologicals | NBP2–43648 |
| Goat anti-rabbit, HRP | Abcam | ab6721 |
| Rabbit anti-mouse, HRP | Sigma-Aldrich | AP160P |
| Goat anti-rabbit, Alexa 488 | Abcam | ab150077 |
| Commercial Assays | ||
| Triglyceride Assay Kit | Biovision | K622–100 |
| Norepinephrine ELISA Kit | Labor Diagnostika Nord | BA E-5200 |
| Other Chemicals | ||
| 6-hydroxydopamine (6-OHDA) | Sigma-Aldrich | 162957 |
| Oil Red O | Alfa Aesar | A12989 |
| DAPI Mounting Medium | Vector Laboratories | H-1200 |
| cDNA Conversion Kit | Quanta Bioscience | 95048–100 |
| SsoFast EvaGreen Supermix | Bio-Rad | 1725202 |
| qPCR Resources | ||
| Primers | RefSeqs | qPCR primer sequences (5’ → 3’) |
| 18s | NR_003278.3 | F: GTAACCCGTTGAACCCCATT R: CCATCCAATCGGTAGTAGCG |
| G6Pase | NM_008061.4 | F: TGGTAGGCAACTGTTTGTTGGTGC R: AGAATCCTGGGTCTCCTTGCCATT |
| PEPCK | NM_011044.2 | F: GCCTGGATGAAGTTTGATGCCCAA R: TTTGTCTTCACTGAGGTGCCAGGA |
| SREBP1c | NM_001313979.1 | F: GCAGCCACCATCTAGCCTG R: CAGCAGTGAGTCTGCCTTGAT |
| ChREBP | NM_021455.4 | F: CCAGCCTCAAGGTGAGCAAA R: CATGTCCCGCATCTGGTCA |
| Pparα | NM_001113418.1 | F: AAGAACCTGAGGAAGCCGTTCTGT R: GCAGCCACAAACAGGGAAATGTCA |
| FAT/CD36 | NM_001159555.1 | F: ATTCCCTTGGCAACCAACCA R: CGTGGCCCGGTTCTAATTCA |
| FATP5 | NM_009512.2 | F: GATGCTTTAGAGCGGCAAGC R: AACTTGGCCAACCCCAGAAA |
| DGAT1 | NM_010046 | F: GTTTCCGTCCAGGGTGGTAG R: GTTGGATCAGCCCCACTTGA |
| DGAT2 | NM_026384 | F: CAGGTGCCGTCTTGGGTTAT R: CAGGAGGATATGCGCCAGAG |