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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Toxicol In Vitro. 2019 Jun 10;60:245–251. doi: 10.1016/j.tiv.2019.06.006

Figure 2. Overview of experimental design.

Figure 2.

(A) Rapid Equilibrium Dialysis (RED) assay following manufacturer protocol to measure percent unbound in plasma; (B) additional protein-free control for RED assay to measure percent equilibration in absence of proteins; (C) alternative method to measure percent unbound in plasma using Solid Phase Micro-Extraction (SPME), adapted from (Musteata et al., 2006).