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Central composite design matrix for the production of fibrinolytic protease from X. oryzae.
| Std | A:pH | B:Sucrose | C:Yeast extract | Enzyme activity (U/g) |
|---|---|---|---|---|
| 1 | −1 | 1 | 1 | 622 |
| 2 | 1 | 1 | 1 | 1590 |
| 3 | 0 | 0 | 0 | 1269 |
| 4 | 1 | 1 | −1 | 2068 |
| 5 | 1.682 | 0 | 0 | 1050 |
| 6 | 0 | 0 | 0 | 1231 |
| 7 | 0 | 0 | 0 | 1290 |
| 8 | 0 | 0 | 0 | 1249 |
| 9 | 0 | 1.682 | 1 | 1500 |
| 10 | 0 | 0 | 1.682 | 1508 |
| 11 | −1 | 1 | −1 | 841 |
| 12 | −1 | −1 | 1 | 2213 |
| 13 | 1 | −1 | −1 | 873 |
| 14 | 0 | 0 | 0 | 1348 |
| 15 | −1 | −1 | −1 | 1372 |
| 16 | 0 | −1.682 | 0 | 2110 |
| 17 | 0 | 0 | −1.682 | 1200 |
| 18 | −1.682 | 0 | 0 | 810 |
| 19 | 0 | 0 | 0 | 1308 |
| 20 | 1 | −1 | 1 | 1480 |
