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. 2019 Aug 30;9:12610. doi: 10.1038/s41598-019-48911-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic overview of La-SSS, which consists of (a) buffer bottle, (b) HPLC pump, (c) manual injector, (d) sample injection syringe, (e) SEC column, (f) HPLC UV detector (g) auto valve, (h) compact UV source, (i) compact UV detector, (j) SAXS cell, (k) waste bottle or fraction collector, and (l) PC that is used for monitoring UV intensity at SAXS cell position and for switching the flow path between a waste bottle and a SAXS cell. The down figure corresponds to the magnified pictures of SAXS cell, compact UV source, and compact UV detector, which are highlighted by pink dotted circle in upper figure. Dark blue and yellow lines correspond to a flow path and an optical fiber, respectively, and the sample flow path was set to 5 mm. Black dotted circle indicates X-ray irradiation zone. The system has another function “stopping mode”. When the OD₂₈₀ value exceeds the set value, the auto valve automatically switches the path from a SAXS cell to a waste bottle, as shown by green arrow. As a result, the solution exceeding the set OD₂₈₀ value, target protein solution, remains in the SAXS cell.