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. 2019 Aug 30;9:12610. doi: 10.1038/s41598-019-48911-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time evolutions of UV intensity at 280 nm from HPLC (green line) (U_h(t)) and SAXS integrated intensity (I_int(t)) (yellow circle) at the flow rate of (a) 0.010 mL/min, (b) 0.020 mL/min, (c) 0.050 mL/min, (d) 0.10 mL/min and (e) 0.20 mL/min obtained by injecting BSA solution with the initial concentration and the volume of 5.4 mg/mL and 500 μl, respectively. Three peaks were observed from UV-HPLC, of which peak positions are highlighted by light green, blue and red arrows from the higher molecular weight component to the lower one.