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. 2019 Aug 30;9:12610. doi: 10.1038/s41598-019-48911-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Time evolutions of integrated SAXS intensity (I_int(t)) (yellow circle) and concentration calculated from U_c(t) (UV-SAXS-cell: red line) by injecting OVA solution with the initial concentration and the volume of 5.0 mg/mL and 500 μl, respectively. We selected two regions; Region 1 (peak at t = 265 min: highlighted by pink dotted rectangle) and Region 2 (peak at t = 155 min: highlighted by light blue rectangle) for obtaining averaged SAXS profiles. (b) Averaged SAXS profiles over Region 1 (pink circle) and Region 2 (light blue circle), respectively. (c) Guinier plots of averaged SAXS profiles from Region 1 (pink, R_g = 23.9 ± 0.4 Å) and Region 2 (light blue, 63.8 ± 28.4 Å), respectively.