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. 2019 Aug 30;9:12610. doi: 10.1038/s41598-019-48911-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Time evolutions of integrated SAXS intensity (I_int(t)) from flow mode (red circle) and stopping mode (blue circle) by applying AF solution with the initial concentration and the volume of 5.1 mg/mL and 500 μl, respectively. We selected two regions; Region 1 (highlighted by green dotted rectangle) and Region 2 (highlighted by pink dotted rectangle) for obtaining averaged SAXS profiles. (b) Averaged SAXS profile from the Region 1 (green circle) in (a) and calculated SAXS profile based on crystal structure (blue curve). Inset shows P(R) calculated from averaged SAXS profile. P(R) from the flow mode is slightly oscillating due to insufficient data quality, as shown by green solid arrows. (c) Averaged SAXS profile from the Region 2 (pink circle) in (a) and calculated SAXS profile based on crystal structure (blue curve). Inset figures show P(R) functions calculated from averaged SAXS profiles.