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. 2019 Aug 30;9:12610. doi: 10.1038/s41598-019-48911-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Time evolutions of integrated SAXS intensity (I_int(t)) (yellow circle) and concentration calculated from U_c(t) (UV-SAXS-cell: red line) by injecting PNGase-PUB solution with the initial concentration and the volume of 2.7 mg/mL and 300 μl, respectively. We selected a region at t = 860 min highlighted by blue dotted rectangle for obtaining averaged SAXS profile. (b) Averaged SAXS profile from the region highlighted by blue dotted rectangle in (a). (c) Guinier plot of averaged SAXS profile (R_g = 15.3 ± 0.5 Å).