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. 2019 Aug 30;9:12609. doi: 10.1038/s41598-019-48090-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glycemia, food intake, body weight and circulating markers. Offspring of HFD mothers showed higher random glycemia (A) and daily food intake (B) at weaning and throughout growth compared to offspring of ND mothers, consistent with a greater body weight starting from 5 weeks of age (C). Circulating levels of insulin and cytokines were not different between groups at weaning (D); at 6 months of age the offspring of HFD mothers showed higher leptin and resistin, and lower insulin and MCP1 levels compared to age-matched controls (E). In line graphs, data are presented as mean ± SEM. In boxplots, black points represent outliers, i.e. cases with values between 1.5 and 3 times the interquartile (IQ) range. *p < 0.05.