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. 2019 Aug 30;9:12609. doi: 10.1038/s41598-019-48090-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Colon microbiota functional analysis. Predictive functional analysis using PICRUSt with Kyoto Encyclopaedia of Genes and Genomes (KEGG) revealed colon microbiota-derived pathways that were differently regulated in the offspring born to mothers fed high-fat versus normal diet, both at 1 (A) and 6 months of age (B). The identified pathways are implicated in metabolism, genetic information processing, environmental information processing, cell motility, endocrine and nervous system and human diseases, and drug development. Pathways with a relative fold-change >10% and a significant difference (p < 0.05) between NDm and HFDm offspring in the Wilcoxon-rank test are shown.