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. 2019 Aug 30;9:12607. doi: 10.1038/s41598-019-48816-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Self-assembly, growth, and viability of 3D EVT spheroids over eight days. (a) EVTs seeded at three different densities form spheroids that increase in size over eight days. 4x magnification. Scale bar indicates 200 µm. (b) Mean spheroid diameter over time across three seeding densities. (c) Immunofluorescent images of live and dead stain using calcein AM and ethidium homodimer-1, respectively. Green colour indicates live cells. Red colour indicates dead cells. 10x magnification. Scale bar indicates 200 µm. (d) Ratio of surface area of live to dead cells at days four, six, and eight. (e) Representative image of H&E staining of a spheroid cross-section. Scale bar indicates 100 µm. n = 3.