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. 2019 Aug 30;9:12614. doi: 10.1038/s41598-019-49062-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferative Capacity (n = 8). (a) Representative images of EdU⁺/DAPI overlay after 48 hours of AGE treatment. EdU⁺ nuclei are pink and have active DNA synthesis. Blue nuclei (DAPI) do not have active DNA synthesis. (b) Graphical representation of EdU images (Panel A). Data represented as ratio of EdU⁺ nuclei to total nuclei. (c) Cell counts after 48 hours of AGE treatment. Dashed line represents initial seeding density of 15,000 cells. (d,e) ddPCR gene transcript counts for Myb-related protein B (Mybl2) and proliferating cell nuclear antigen (Pcna). (f) Cytostatic MTT shown as absolute arbitrary absorbance units. *p < 0.0125 main effect for AGE vs. AGE-0 μg/ml, mixed effects regression. NG:HG p < 0.0125 indicates main effect for glucose condition, mixed effects regression. Data presented as mean ± standard error. ■ Normal Glucose (NG), High Glucose (HG).

Inline graphic — Proliferative Capacity (n = 8). (a) Representative images of EdU⁺/DAPI overlay after 48 hours of AGE treatment. EdU⁺ nuclei are pink and have active DNA synthesis. Blue nuclei (DAPI) do not have active DNA synthesis. (b) Graphical representation of EdU images (Panel A). Data represented as ratio of EdU⁺ nuclei to total nuclei. (c) Cell counts after 48 hours of AGE treatment. Dashed line represents initial seeding density of 15,000 cells. (d,e) ddPCR gene transcript counts for Myb-related protein B (Mybl2) and proliferating cell nuclear antigen (Pcna). (f) Cytostatic MTT shown as absolute arbitrary absorbance units. *p < 0.0125 main effect for AGE vs. AGE-0 μg/ml, mixed effects regression. NG:HG p < 0.0125 indicates main effect for glucose condition, mixed effects regression. Data presented as mean ± standard error. ■ Normal Glucose (NG), High Glucose (HG).