Fig. 1.

Gating strategy applied to identify CD4/CD25/Foxp3 positive cells. Lymphocytes isolated from whole blood by Ficoll-Paque Plus gradient method were washed, gated in their totality (A, gate R1) and singlets (B, gate R2) for the identification of CD4 positive cells (C, E and G). Finally, CD4⁺ cells (gate R3) were plotted for CD25 and Foxp3 (D, F and H). Dot plots values indicated the percentage of positively stained cells in the different experimental conditions. FACS pictures are representative of six samples with similar results. Data are presented as means ± SD of n = 6 mice per group.