Value of the data •This data describes the chemical characterization of novel indirubin derivative, LDD-1819, which modulates cell plasticity and inhibits tumorigenicity. •This data provides further characterization of the biological activity of compound LDD-1819. •Overall, this data should allow the scientific community to ascertain the potential utility of LDD-1819 for development as an anti-cancer agent or chemical tool to facilitate cell reprogramming protocols.