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. 2019 Aug 30;9:12603. doi: 10.1038/s41598-019-48913-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the numbers of sequences included in MSAs. The X-axis shows the numbers of sequences in MSAs generated using the sequence database of 2011; the Y-axis shows those using the sequence database of 2016. Monomers in the Duarte dataset were compared. Dots show each monomer. Blue dots represent both 2011 and 2016 MSAs satisfying the diversity requirement of PSICOV. Green dots represent only 2016 MSAs satisfying the requirement. Pink dots represent neither 2011 nor 2016 MSAs satisfying the criterion.