BTSL are uniquely found in dicotyledonous plants. (A) Domain organization of the BTSL and BTS proteins and closest mammalian homologs. (B) Phylogenetic tree of BTS homologs from selected plant species. Sequences were found by BLASTing the amino acid sequences of Arabidopsis BTS, BTSL1, BTSL2, and rice HRZ1 in Ensembl Plants (http://plants.ensembl.org). Species used: Amborella, Amborella trichocarpa; At, A. thaliana; Bo, Brassica oleracea; Hv, Hordeum vulgare; Mt, Medicago truncatula; Os, Oryza sativa; Pp, Physcomitrella patens; Pt, Populus trichocarpa; Sl, Solanum lycopersicum; Ta, Triticum aestivum. Numbers next to branches indicate bootstrapping values for 100 replications. The scale bar indicates the rate of evolutionary change expressed as number of amino acid substitutions per site.