Fig. 1.

Flow LD confirms B-DNA and indicates shorter persistence length in diglyme. (A) DNA LD spectra (720 rpm; see SI Appendix, Figs. S14–S16 for all speeds) with wavelength-independent LD^r is consistent with bases near perpendicular to helix axis in diglyme and glycerol. (B) LD^r in diglyme is less negative than in glycerol of the same viscosity. Hence, DNA persistence length is shortened in diglyme. Extrapolation to zero flow gradient indicates persistence length in diglyme between 70 and 81% of that in glycerol (see text and SI Appendix, section 1d).