Fig. 5.

Effect of calcitriol treatment on body weight, plasma calcitriol, calcium, and phosphorus in WT and Folr1 KO mice. (A) Significant weight loss was observed at days 4 and 6 following treatment with calcitriol (2.5 μg/kg) compared with vehicle (corn oil). Calcitriol treatment also increased calcitriol (B) and calcium (C) concentrations in mouse plasma, but not phosphorus (D). Results are presented as mean ± SEM for n = 3 independent experiments, with at least 4 animals per group. Asterisks represent data points significantly different from vehicle control (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). For weight data, the pound symbol represents data points significantly different from day 0 (time before vehicle or calcitriol injections) (^#P < 0.05; ^####P < 0.0001).