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. 2019 Aug 12;116(35):17355–17360. doi: 10.1073/pnas.1901653116

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Fig. 2. — Kinetochores retain Mps1 when attached to microtubule tips. (A) Fluorescence image of kinetochore particles (from SBY15285) carrying Mps1-SNAP 549 (cyan) and Mtw1-CLIP 647 (red) tethered to a coverslip and exposed to free, Taxol-stabilized Alexa Fluor 488 microtubules (green). By thermal diffusion, the filaments attached via their tips to the coverslip-tethered kinetochores. Colors are offset vertically; cyan-red pairs are colocalized, dual-color kinetochore particles. (B) Percentages of kinetochore particles retaining Mps1. Bars show mean ± SD values from 28 images of 40 tip-attached particles and 366 coverslip-tethered particles. Histograms show the corresponding distributions of colocalization measured for the tip-attached and coverslip-tethered particles in each individual image.