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. 2019 Aug 31;16:174. doi: 10.1186/s12974-019-1559-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The effects of silencing BI-1 on the NPR-CYP complex at 72 h post-HI. Representative bands of western blot data (a). Quantification of western blot bands for BI-1 (b) and P4502E1 (c) (data expressed as mean ± SD; an asterisk indicates p < 0.05 vs sham; number sign p< 0.05 vs vehicle; @ p< 0.05 vs Ad-TMBIM6 or scramble; n = 6/group using one-way ANOVA followed by Tukey’s multiple-comparison post hoc analysis). Immunofluorescence staining of Iba-1 with BI-1 or P4502E1 (d, e). Green was for microglial staining, red was for BI-1 or P4502E1 staining, and blue was for DAPI. Merge (yellow) showed the co-localization of BI-1 or P4502E1 on microglia (scale bar = 50 μm; n = 3/group)