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. Author manuscript; available in PMC: 2019 Aug 31.
Published in final edited form as: Cell Metab. 2018 Aug 30;29(1):78–90.e5. doi: 10.1016/j.cmet.2018.08.002

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Mouse monoclonal anti-VDAC Abcam Cat#ab14734; RRID: AB_443084
Rabbit monoclonal anti-VDAC Cell Signaling Technology Cat#D73D12; RRID: AB_10557420
Mouse Monoclonal total OXPHOS rodent antibody cocktail Abcam Cat#ab110413; RRID: AB_2629281
Mouse monoclonal anti-NDUFAl Yadava et al., 2002 N/A
Mouse monoclonal anti-OPA1 BD Biosciences Cat#612606; RRID: AB_399888
Fluorescent TrueBlot: Anti-Mouse Ig DyLight 800 Rockland Cat#18–4517-32; RRID: AB_2610842
Rabbit IgG (H&L) Antibody DyLight 800 Conjugated Pre-Adsorbed Rockland Cat#611–145-122; RRID: AB_1057618
Critical Commercial Assays
Ion Total RNA-Seq Kit v.2 Life Technologies Cat#4475936
Ion PI Sequencing 200 Kit v.2 chemistry Life Technologies Cat#4485149
PureLink RNA-Easy kit Life Technologies Cat#12183018A
RiboMinus Eukaryote System v.2 Life Technologies Cat #A15026
OxiSelect Nitrotyrosine ELISA Kit Cell Biolabs Cat#STA-305
CaspaseGlo 3/7 Promega Cat#G8090
Deposited Data
Raw sequence files and associated metadata This paper SRA: SUB2425516
Reproducible code for generating the values presented in this paper This paper https://github.com/chop-dbhi/mcmanus_ant1
Experimental Models: Organisms/Strains
Mouse: WT: C57Bl/6JEiJ Navarro et al., 2012 N/A
Mouse: COI: COIV421A C57Bl/6JEiJ This paper N/A
Mouse: ND6: ND6P25L C57Bl/6JEiJ This paper N/A
Mouse: ANT1: Ant1−/− C57Bl/6JEiJ Morrow et al., 2017 N/A
Mouse: ANT1/COI: Ant1−/−, COIV421A C57Bl/6JEiJ This paper N/A
Mouse: ANT1/ND6: Ant1−/−, ND6P25L C57Bl/6JEiJ This paper N/A
Oligonucleotides
Primers for mtDNA LT-PCR ND2 Forward: 5’CTGGAA TTCAGCCTACTAGCAATTATCC-3’; 12s Reverse:
5’-TTTAGGTTTATGGCTAAGCATAGTGGGG- 3’ 		This paper N/A
Primers for mtDNA deletions flanking multiple TaqI restriction sites: Deletion Forward: 5’-AGGCCAC CACACTCCTATTG-3’, position 8810–8829;
Deletion Reverse: 5’-AATGCTAGGCGTTTGATTGG-3’, position 13098–13117		 Vermulst et al., 2008b N/A
Primers for mtDNA Taq1 controls: Control Forward: 5’-TCGGCGTAAAACGTGTCAAC-3’, position 350–369; Control Reverse: 5’-CCGCCAAGTCCTTTGAGTTT-3’ position 579–598 Vermulst et al., 2008b N/A
Software and Algorithms
Speckle tracking echocardiography: Vevostrain software Visual Sonics https://www.visualsonics.com/product/software/vevo-lab
Electron micrograph quantification: Image J National Institutes of Health https://imagej.nih.gov/ij/download.html
Western Analysis: Image Studio LI-COR https://www.licor.com/bio/products/software/image_studio_lite/