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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Dev Biol. 2019 Jun 21;454(1):15–20. doi: 10.1016/j.ydbio.2019.06.016

Figure 1 – FNDC-1 is expressed in spermatids and targeted to mitochondria.

Figure 1 –

mRuby3 red fluorescent protein sequence was inserted into the N-terminal genomic coding region of fndc-1 using CRISPR-Cas9 editing and HDR.

Images in panels A - D contain both fluorescence (left) and differential contrast interference (right).

A Intestine, body wall muscles, and pharynx of males and hermaphrodites, as labeled. Scale bar: 100 μm.

B Hermaphrodite spermatheca. Note the lack of expression in oocytes (arrows) compared to spermatids (arrowheads). Scale bar: 20 μm.

C Male gonad. Scale bar: 20 μm.

D Isolated spermatids from males expressing mRuby3::FNDC-1. Scale bar: 5 μm.

E Higher magnification image of mRuby3::FNDC-1 in spermatids.

F Genomic single copy CRISPR-Cas9 modified ucr-2.3::GFP in the same spermatids as panel E.

G Overlay of mRuby3::FNDC-1 (red) and ucr-2.3::GFP (green). Scale bar: 5 μm.