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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Cell Signal. 2019 Jul 25;63:109366. doi: 10.1016/j.cellsig.2019.109366

Figure 6. Co-immunoprecipitation of wild-type MELK1−340 with arrestin-31–393.

Figure 6.

(A) Western analysis of coimmunoprecipitation. HEK293 arrestin-2/3 knockout cells were transfected with HA-arrestin-31−393 (1 μg), FLAG-MELK1−340 (1 μg), or both plasmids encoding arrestin-3 and MELK at indicated DNA ratios for 48 h prior to immunoprecipitation with FLAG primary antibody. Western analysis was performed using anti-arrestin (1:10,000; F431) and anti-FLAG (1: 1,000; F3165 Sigma) antibodies. (B) Arrestin-31−393 co-immunoprecipitation with MELK1−340 was quantified using densitometric analysis of arrestin-3 blots. Non-specific arrestin-3 binding (no bait control) is also shown. Statistical analysis was performed using One-way ANOVA followed by Tukey’s post hoc test with correction for multiple comparisons (*, p<0.05).