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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Dev Biol. 2019 Jun 18;454(1):44–51. doi: 10.1016/j.ydbio.2019.06.011

Figure 5. Exogenous FGF beads are sufficient to recruit progenitor cells.

Figure 5.

A) Stem cells marked by the transgene lhx1a:eGFP are not attracted to BSA soaked agarose beads after 24 hours of culture. (B) Beads soaked with Fgf4 are more closely surrounded by renal stem cells, indicated by a decrease in cell to bead distance from an average of 28.8μm to 17.75μm. (C) Beads soaked with FGF10 strongly attract renal stem cells which accumulate adjacent to the bead at a mean distance of 3.42μm. (D) Quantification of cell to bead distances after 24 hours of culture demonstrate a chemoattractive role of FGF ligands on the recruitment renal stem cells. 3 biological replicates per condition. Error bars indicate ±SD. Comparisons to BSA beads were made by Kruskal-Wallis test. ns indicates not-significant, starred comparison indicates p=0.008. In Z-projection panels (A-C) scale bar is 25μm.