Skip to main content
. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Hepatology. 2019 Jun 24;70(3):995–1010. doi: 10.1002/hep.30691

Figure 2. Pharmacological activation of PXR sensitizes mice to HS-induced hepatic injury.

Figure 2.

(A) Schematic representation of the PCN pre-treatment model. WT and Pxr−/− mice were intraperitoneally injected with PCN (40 mg/kg per day) for two days before receiving the sham surgery or HS/R. (B and C) The hepatic mRNA (B) and protein (C) expression of Cyp3a11 and Cyp2b10 were measured by qRT-PCR and Western blotting, respectively. (D) The liver microsomal productions of 1’-OH midazolam (left) and noroxycodone (right) were measured by UPLC-MS analysis. (E) Liver histology was analyzed by H&E staining. Bar is 100 μm. Shown below are quantification of necrotic areas and Suzuki scores. (F) Serum levels of ALT. n=4~5 for each group. *, p < 0.05; **, p < 0.01, the comparisons are labeled (B, D and F), or compared to the WT+VEH group (E).