(A) Liver histology was analyzed by H&E staining in VEH- or NACA-treated VP-PXR mice that were subject to the sham surgery or HS/R. Bar is 100 μm. (B to D) Mice are the same as in (A). Shown are quantification of necrotic areas (B), Suzuki scores (C), and serum levels of ALT (D) in mice subject to HS/R. (E) Mice are the same as in (A). The level of 4-HNE was measured by immunostaining with the positive staining indicated by arrowheads. Bar is 100 μm. n=4~5 for each group. *, p < 0.05; **, p < 0.01, compared to the VEH group (B and C), or the comparisons are labeled (D).