Intravascular S. mansoni cleave polyP. Pi level (µM, mean ± SD) detected 2, 4 and 24 hours (h) after incubating groups of ~1000 living schistosomula (grey bars), individual adult female (red bars) or adult male (blue bars) worms in 2 mM polyP. At least 10 separate male or female parasites were used per assay. Individual males generate statistically significantly more Pi compared to individual females at all comparable timepoints (Two-way ANOVA, * p < 0.05, ** p < 0.001).