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. 2019 Feb 28;104(9):1789–1797. doi: 10.3324/haematol.2017.179937

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Figure 4. — Induction of neutrophilic differentiation in UBE2A wild-type (WT) or I33M 32Dcl3 cell line. Cells were treated with IL-3 or granulocyte-colony stimulating factor (GCSF). (A) Fluorescence-activated cell sorting analysis (FACS) analysis of CD11b staining after induction of differentiation at days 3 and 6. (B) CD11b immunofluorescence staining for 32Dcl3 control (CTRL), UBE2A WT and I33M at day 6 showing a clear reduction in UBE2A I33M CD11b staining (scale bar: 20 μm).